Significance of cytokeratin gene (CK-20 mRNA) expression in metastatic lymph nodes in colon carcinoma patients.
- Author:
Xin YE
1
;
Yi LI
;
Gang HOU
;
Zhongmin LIU
;
Tingwu CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Colonic Neoplasms; diagnosis; genetics; pathology; Female; Gene Expression; Genetic Markers; genetics; Humans; Intermediate Filament Proteins; genetics; Keratin-20; Lymphatic Metastasis; diagnosis; Male; Middle Aged; Neoplasm Staging; Prognosis; RNA, Messenger; biosynthesis; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Oncology 2002;24(3):261-263
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo elucidate the significance of detecting cytokeratin gene (CK-20 mRNA) expression in metastatic lymph nodes of colon carcinoma patients.
METHODSThe expression of CK-20 mRNA was detected in 342 lymph nodes by reverse transcription-polymerase chain reaction (RT-PCR) in 49 colon carcinoma patients. Its incidence was compared with that by routine pathologic examination.
RESULTSThe positive rates of detecting metastasis in the lymph nodes were 21.9% by RT-PCR and 11.1% by routine pathologic examination.
CONCLUSIONThe detection of cancer metastasis in the lymph nodes in colon carcinoma is almost doubled (21.9% vs 11.1%) by CK-20 mRNA, which may provide a guiding significance in staging, treatment planning and prognosis in the prognostic estimation of colon carcinoma patients.
