Gene expression profile of human hepatocellular carcinoma cell lines with different metastatic potentials.
- Author:
Yan LI
1
;
Zhaoyou TANG
;
Shenglong YE
;
Binbin LIU
;
Yinkun LIU
;
Jie CHEN
;
Qiong XUE
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Northern; Carcinoma, Hepatocellular; genetics; pathology; Gene Expression; Gene Expression Profiling; Humans; Liver Neoplasms; genetics; pathology; Neoplasm Metastasis; genetics; Oligonucleotide Array Sequence Analysis; RNA, Neoplasm; analysis; Tumor Cells, Cultured
- From: Chinese Journal of Oncology 2002;24(6):533-536
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare gene expression profile of human hepatocellular carcinoma (HCC) cell lines with different metastatic potentials, so as to screen for metastasis-related genes.
METHODSGene expression profile of MHCC97-L and HCCLM3, two HCC cell lines with similar genetic background but different in spontaneous metastatic potentials, were studied by cDNA microarray.
RESULTSFrom 1,626 screened genes, 25 differentially expressed genes were found, 18 showed decreased expression including the decreased expression of cell cycle control genes Rb2, mismatch repair gene hMSH2, and signal transduction gene protein kinase C beta 2 and 7 increased expression including signal transduction gene MAP kinase kinase 6, cell proliferation gene E25, immunity related gene SP40, 40, etc in HCCLM3.
CONCLUSIONThe genes, being closely associated with cancer metastasis, could be considered as potential markers to predict metastasis and targets for anti-metastasis intervention.
