Screening for inhibitor of vascular endothelial growth factor from random peptide library.
- Author:
Jie WU
1
;
Hongbin ZHANG
;
Jie WANG
;
Taicheng YANG
;
Jiang XIAN
;
Chuanhong YANG
;
Wenling ZHENG
;
Huipeng CHEN
;
Qingming WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Binding Sites; Endothelial Growth Factors; antagonists & inhibitors; metabolism; Enzyme-Linked Immunosorbent Assay; Humans; Intercellular Signaling Peptides and Proteins; metabolism; Lymphokines; antagonists & inhibitors; metabolism; Peptide Library; Peptides; pharmacology; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factors
- From: Chinese Journal of Oncology 2002;24(6):540-543
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen for the inhibitor of vascular endothelial growth factor (VEGF) 165 from random peptide library.
METHODSPositive phage clones were rescued after two rounds of panning and competitive elution. Its affinity activity to KDR was monitored through ELISA, immunohistochemical method, Chicken CAM assay and MTT.
RESULTSFive specific binding positive target molecule phage clones were obtained which were able to bind to cells whose surface had high KDR, among which, clone 3 and 13 could effectively block the vascularization of the chorioallantoic membrane of chick embryo, but they were not inhibitive on the proliferation of high KDR expression cells.
CONCLUSIONThe peptides, being the inhibitors of VEGF, may be useful in the treatment of cancers.
