Relationship between cytochrome c-mediated caspase-3 activity and chemoresistance in cisplatin-resistant human ovarian cancer cell lines.
- Author:
Xiaokui YANG
1
;
Fang ZHENG
;
Hui XING
;
Qinglei GAO
;
Wei WANG
;
Yunping LU
;
Shixuan WANG
;
Ding MA
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Apoptosis; Caspase 3; Caspases; metabolism; Cisplatin; pharmacology; Cytochrome c Group; metabolism; Drug Resistance, Multiple; physiology; Drug Resistance, Neoplasm; physiology; Female; Humans; Ovarian Neoplasms; pathology; Proto-Oncogene Proteins c-bcl-2; metabolism; Tumor Cells, Cultured; bcl-X Protein
- From: Chinese Journal of Oncology 2002;24(6):544-547
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of anti-apoptosis gene (bcl-X(L)), cytochrome c and caspase-3 activity on chemoresistance in cisplatin-resistant human ovarian cancer cell lines (A2780/DDP, COC1/DDP).
METHODSThe expression of bcl-X(L) cisplatin treated cytochrome c and caspase-3 activity were monitored by RT-PCR and Western blot in cisplatin-resistant (A2780/DDP, COC1/DDP) and cisplatin-sensitive (A2780, COC1) cell lines. The apoptotic rates of A2780, COC1, A2780/DDP and COC1/DDP were detected with flow cytometry after having been treated by cisplatin.
RESULTSThe expression of bcl-X(L) in A2780/DDP and COC1/DDP was significantly higher than that in A2780 and COC1 cells, whereas the expression of cytochrome c, caspase-3 activity and apoptotic rates of A2780/DDP and COC1/DDP were significantly reduced more than those of A2780 and COC1 after having been treated by cisplatin (P < 0.05).
CONCLUSIONThe overexpression of anti-apoptotic gene bcl-X(L), which downregulates cytochrome c and decreases caspase-3 activity, may be related to cisplatin-resistance in human ovarian cancer cell lines.
