Correlation between epidermal growth factor and overexpression of vascular endothelial growth factor in hepatocellular carcinoma.
- Author:
Gengwen HUANG
1
;
Lianyue YANG
;
Jianqing YANG
;
Heli LIU
;
Zhili YANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Carcinoma, Hepatocellular; metabolism; Endothelial Growth Factors; analysis; Epidermal Growth Factor; analysis; Female; Humans; Intercellular Signaling Peptides and Proteins; analysis; Liver Neoplasms; metabolism; Lymphokines; analysis; Male; Middle Aged; Neovascularization, Pathologic; Statistics as Topic; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factors
- From: Chinese Journal of Oncology 2002;24(6):564-566
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the correlation between epidermal growth factor (EGF) and overexpression of vascular endothelial growth factor (VEGF) in hepatocellular carcinoma (HCC).
METHODSExpressions of EGF, VEGF and microvessel density were studied through immunohistochemical SABC method in 36 HCC specimens, their paraneoplastic liver tissues and 6 normal liver tissues with the correlation between these parameters analyzed. Recombinant human EGF was used to stimulate HepG(2) cells and semi-quantitative reverse transcription PCR was adopted to detect the expression of VEGF in HepG(2) cells.
RESULTSThe positive rates of EGF and VEGF expression in HCC tissue were 75.0% and 88.9%. There was positive correlation between EGF and VEGF expression (P < 0.01, r = 0.462). Recombinant human EGF could induce the expression of VEGF in HepG(2) cells in a dose and time dependent manner.
CONCLUSIONThe expression of EGF in HCC underlies the overexpression of VEGF in HCC.
