Preliminary study on the mechanism of connexin 43 gene transfection in the control of glioma cell proliferation.
- Author:
Zhi-bai XIA
1
;
Pei-yu PU
;
Qiang HUANG
;
Yong-ping YOU
;
Guang-xiu WANG
;
Chun-yan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Cell Division; physiology; Connexin 43; genetics; physiology; DNA, Complementary; genetics; Glioma; pathology; Rats; Transfection; Tumor Cells, Cultured
- From: Chinese Journal of Oncology 2003;25(1):4-8
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the mechanism involved in the control of glioma cell proliferation with transfection of connexin (Cx) 43 gene.
METHODSC6 rat glioma and TJ905 human glioblastoma cell lines without Cx43 gene expression were transfected with Cx43cDNA mediated by lipofectamine. Northern blot, in situ hybridization and immunohistochemical technology were used to detect the expression of Cx43mRNA and its protein with MTT assay and silver colloid stain for the detection of cell proliferation, TUNEL method for determination of cell apoptosis, scrape loading dye transfer (SLDT) for GJIC, Western blot and immunohistochemical technology for bFGF, PDGF, EGFR, IGF-I and IGFBP3 expression.
RESULTSCx 43 gene transfected glioma cells showed decreased proliferation, restored GJIC and decreased bFGF, PDGF, IGFBP3, except EGFR expression and cell apoptosis which showed no change.
CONCLUSIONThe mechanism of Cx 43 gene inhibiting gliomas cell proliferation is the restoration of GJIC and decreased autocrine growth factors.
