Differential expression of RPL6/Taxreb107 in drug resistant gastric cancer cell line SGC7901/ADR and its correlation with multiple-drug resistance.
- Author:
Jing-ping DU
1
;
Xiao-hang JIN
;
Yong-quan SHI
;
Yun-xin CAO
;
Yan-qiu ZHAO
;
Chang-Jiang LIU
;
Fang YIN
;
Wen-hua HU
;
Bao-jun CHEN
;
Tai-dong QIAO
;
Dai-ming FAN
Author Information
- Publication Type:Journal Article
- MeSH: DNA-Binding Proteins; metabolism; Drug Resistance, Multiple; physiology; Drug Resistance, Neoplasm; physiology; Humans; Statistics as Topic; Stomach Neoplasms; pathology; Tumor Cells, Cultured
- From: Chinese Journal of Oncology 2003;25(1):21-25
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the differential expression of RPL6/Taxreb107 between drug-resistant gastric cancer cell line SGC7901/ADR and gastric cancer cell line SGC7901 as well as its correlation with multiple-drug resistance (MDR) in gastric cancer cells.
METHODSTotal RNA was extracted from SGC7901 and SGC77901/ADR, with internal control RT-PCR, Northern blot, gene cloning and expression, construction of eukaryotic expression vector, gene transfection by electroporation. The accumulation and retention of ADR in transiently transfected cell was detected by flow cytometry.
RESULTSThe internal control RT-PCR and Northern blot showed high RPL6/Taxreb107 expression in SGC7901/ADR cell line. Sense and antisense eukaryonic expression vectors demonstrated by double enzyme digestion were successfully transfected into gastric cancer cell line SGC7901 and SGC7901/ADR respectively by electroporation. The accumulation and retention of ADR detected 48 hours after transfection showed that RPL6 gene had shown effect on drug resistance in gastric cancer cell.
CONCLUSIONThe high expression of RPL6/Taxreb107 in drug resistant gastric cancer cell shows its correlation with multiple-drug resistance in gastric cancer.
