Differential expression of the epithelial membrane protein 1 of laryngeal carcinoma.
- Author:
Ye-hai LIU
1
;
Ping-zhang TANG
;
Zhen-gang XU
;
Yong-fa QI
;
Fang DING
;
Li-yong ZHANG
;
Hai-tao WANG
;
Zhi-hua LIU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Female; Humans; Laryngeal Neoplasms; genetics; metabolism; Male; Middle Aged; Neoplasm Proteins; Oligonucleotide Array Sequence Analysis; Receptors, Cell Surface; biosynthesis; genetics; Reverse Transcriptase Polymerase Chain Reaction
- From: Acta Academiae Medicinae Sinicae 2003;25(1):47-51
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEIn order to explore pathogenic mechanism of laryngeal carcinoma, the involved genes were identified in larynx carcinogenesis by comparing the gene expression profile in matched primary normal epithelial cells and primary laryngeal carcinoma cells from the same patients.
METHODSA cDNA microarray analysis consisting of 11,431 human genes revealed significant changes in the expression of 35 genes, with 8 genes being up-regulated and 27 being down-regulated. The epithelial membrane protein-1 (EMP-1) is one of the down-regulated genes. EMP-1 expression in various kinds of laryngeal carcinoma was determined by semi-quantitative reverse transcriptase polymerase chain reaction (qRT-PCR).
RESULTSThe EMP-1 mRNA levels in all laryngeal carcinoma cells was significantly lower than that in the matched primary normal epithelial cells (P < 0.05) and were not correlated to the stage and differentiation of laryngeal carcinoma (P > 0.05).
CONCLUSIONSThe EMP-1 expression was correlated to larynx carcinogenesis and may be helpful to elucidate the pathogenic mechanism in laryngeal carcinoma.
