Cloning, expression and purification of neural specific HuD cDNA.
- Author:
Jian-hua CHEN
1
;
Xiu-qin LIU
;
Yu-pu GUO
;
Bin ZHANG
;
Yan ZHOU
;
Xiao-yan HU
;
Jian-gang YUAN
;
Bo-qin QIANG
;
Xiao-zhong PENG
Author Information
- Publication Type:Journal Article
- MeSH: Antibodies, Antinuclear; biosynthesis; genetics; isolation & purification; Carcinoma, Small Cell; genetics; immunology; metabolism; Cloning, Molecular; DNA, Complementary; genetics; ELAV Proteins; ELAV-Like Protein 4; Humans; Lung Neoplasms; genetics; immunology; metabolism; Nerve Tissue Proteins; biosynthesis; genetics; isolation & purification; Neurons; immunology; Paraneoplastic Syndromes, Nervous System; genetics; immunology; metabolism; RNA-Binding Proteins; biosynthesis; genetics; isolation & purification
- From: Acta Academiae Medicinae Sinicae 2002;24(3):250-253
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo prokaryoticly express and purify HuD protein and its RNA recognition motifs.
METHODSHuD protein was prokaryoticly expressed and purified by molecular cloning technology. Its biologic activity was testified by Western Blot.
RESULTSPurified HuD protein and its RNA recognized motifs were observed.
CONCLUSIONSThe result might aid for basic research and clinical application.
