Experimental research in vitro of TK/GCV system for osteosarcoma MG-63 cell damage.
- Author:
Hua-Dong ZHANG
;
Zhi LU
;
Yi FENG
;
Xiao-Li LIU
;
Hui-Ming HOU
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Bone Neoplasms; enzymology; genetics; physiopathology; Bystander Effect; drug effects; Cell Line, Tumor; Cell Survival; drug effects; Ganciclovir; toxicity; Humans; Osteosarcoma; enzymology; genetics; physiopathology; Thymidine Kinase; genetics; metabolism; toxicity
- From: China Journal of Orthopaedics and Traumatology 2014;27(3):240-243
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the killing effects of the liposome-mediated thymidine kinase (TK)/ganciclovir (GCV) system on MG-63 osteosarcoma (OS) cells and its bystander effects.
METHODSLiposome-mediated TK gene transfected into MG-63 OS cells, the efficiency of transfection was analyzed by flow cytometry and observed under inverted fluorescence microscope. Non-transfected osteosarcoma MG-63 cells were divided into three groups,in the experimental group 1 transfected TK/GCV cells cultured in solutiona liquid mixture by supernatant by 1/10,1/7,1/5,1/2 ratio to original broth; in the experimental group 2 transfected cells cultured in solutiona liquid mixture of supernatant filtered through 0.22 microm filter by 1/10,1/7, 1/5, 1/2 ratio to original broth, in control group the transfection cells cultured in original culture solution. Cell growth inhibition rate and osteosarcoma cell sensitivity to TK/GCV system were measured by MTT assay in each group.
RESULTSThe TK gene was transfected into MG-63 OS cells successfully by liposome-mediated, flow cytometry instrument detection TK gene transfection cell transfection efficiency can reach 75.5%. Six days later the MTT assay showed that in the experimental group 1 inhibition rate of all concentration ratio of the mixed culture fluid were statistically significant as compared with the control group (P < 0.05), and in the experimental group 2 that of the 1/10 and 1/7 of concentration ratio of mixed culture medium was not statistically significant as compared with the control group (P > 0.05). TK gene transfected MG-63 cells increased with the the GCV concentration,the cell apoptosis rate increased.
CONCLUSIONThe experiment demonstrated that the MG-63 OS cells are sensitive to the liposome-mediated TK/GCV system and bystander effects are significant.