Detection of specific interferon-gamma-secreting T cell response to Mycobacterium tuberculosis RD1-encoded antigens in pleural effusions, ascites, and cerebrospinal fluid.
- Author:
Li-Fan ZHANG
1
;
Xiao-Qing LIU
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, Bacterial; genetics; Ascites; metabolism; Bacterial Proteins; Humans; Interferon-gamma; cerebrospinal fluid; metabolism; Leukocytes, Mononuclear; Mycobacterium tuberculosis; Peptides; Pleural Effusion; immunology; Recombinant Proteins; T-Lymphocytes; metabolism; Tuberculosis, Pulmonary; diagnosis
- From: Acta Academiae Medicinae Sinicae 2009;31(4):438-442
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect Mycobacterium tuberculosis RD1-encoded antigens-specific, interferon-gamma (INF-gamma)-secreting T cells in pleural effusions, ascites, and cerebrospinal fluid.
METHODThe early secretory antigenic target-6 (ESAT-6) and culture filtrate protein-10 (CFP-10) peptides-specific T cells in peripheral blood mononuclear cell (MC), ascites MC, pleural effusions MC, and cerebrospinal fluid MC were detected using enzyme-linked immunospot assay (ELISPOT) for INF-gamma.
RESULTSESAT-6 or CFP-10 peptides-specific, INF-gamma-secreting T cells were detected in peripheral blood, ascites, pleural effusions, and cerebrospinal fluid, which marked the presence of tuberculosis infection. Patients with positive ELISPOT results of INF-gamma-release assay were all diagnosed as active tuberculosis. Spot forming cells in ascites and pleural effusions were much higher than those in peripheral blood (up to 6.4 and 31.9 times).
CONCLUSIONDetection of RD1-encoded antigens-specific, INF-gamma-secreting T cells in pleural effusions, ascites, and cerebrospinal fluid provides a new way to diagnose tuberculosis infection.
