Sensitivity of interferon-gamma release assay T-SPOT. TB in diagnosing extrapulmonary tuberculosis.
- Author:
Fei-Fei HUO
1
;
Li-Fan ZHANG
;
Xiao-Qing LIU
Author Information
- Publication Type:Journal Article
- MeSH: Humans; Interferon-gamma; Interferon-gamma Release Tests; Leukocytes, Mononuclear; Sensitivity and Specificity; Tuberculosis; diagnosis
- From: Acta Academiae Medicinae Sinicae 2009;31(4):449-452
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the sensitivity of an interferon-gamma release assay T-SPOT. TB in the diagnosis of bacteriologically or histologically confirmed extrapulmonary tuberculosis.
METHODTotally 31 patients with bacteriologically or histologically confirmed extrapulmonary tuberculosis in Peking Union Medical College Hospital received T-SPOT. TB assay to detect early secreting antigen target 6 or culture filtrate protein 10 peptides-specific T cells in the peripheral blood mononuclear cells (PBMCs).
RESULTST-SPOT. TB assay showed positive results in 29 patients with extrapulmonary tuberculosis and the sensitivity was 93.5% (95% CI 84.8% - 100%). The median of spot forming cells (SFCs) in response to early secreting antigen target 6 peptides was 196/10(6) PBMCs (interquartile range, 72-532/10(6) PBMCs), the median of SFCs in response to culture filtrate protein 10 peptides was 276 SFCs/10(6) PBMCs (interquartile range, 72-568/10(6) PBMCs), and the median of the incorporate SFCs was 612/10(6) PBMCs (interquartile range, 192-1 152/10(6) PBMCs).
CONCLUSIONT-SPOT. TB is highly sensitive in diagnosing extrapulmonary tuberculosis.
