Application of fluorescence in situ hybridization and flow cytometry in the measurement of telomere length in Chinese patients with bone marrow failure syndrome.
- Author:
Bo LIU
1
;
Wei CUI
;
Bing HAN
;
Xuan WANG
;
Jie LIN
;
Yong-Qiang ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Anemia, Aplastic; Bone Marrow Diseases; Case-Control Studies; Female; Flow Cytometry; methods; Hemoglobinuria, Paroxysmal; genetics; Humans; In Situ Hybridization, Fluorescence; methods; Male; Middle Aged; Telomere; genetics; Young Adult
- From: Acta Academiae Medicinae Sinicae 2009;31(5):547-550
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo measure the telomere length in patients with bone marrow failure syndrome (BMF) using fluorescence in situ hybridization and flow cytometry (Flow-FISH).
METHODSBlood samples were collected from 8 patients with BMF. Telomere lengths of mononuclear cells from 8 BMF patients and granulocytes from 3 of these 8 BMF patients (all these 3 patients were suffered from aplastic anemia) were measured using Flow-FISH, and the results were compared with those of normal controls. Telomere lengths were measured by Southern blot in 3 patients, and the results were compared with those obtained from Flow-FISH.
RESULTSAmong these 8 BMF patients, 5 have decreased telomere length in their mononuclear cells. However, different white cell subgroups, specifically mononucleated cells and granulocytes, have different degree of telomere shortening. In 3 BMF patients who underwent both Flow-FISH and Southern blot, 2 patients had consistent measurement results.
CONCLUSIONFlow-FISH can be used for the measurement of telomere length in Chinese patients with BMF.
