Comparison of modified Bethesda assay and Nijmegen assay in detecting FVII inhibitor in patients with hemophilia A.
- Author:
Lian-Kai FAN
1
;
Zhi-Wei WANG
;
Bao-Lai HUA
;
Wei SU
;
Shu-Jie WANG
;
Yong-Qiang ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Autoantibodies; blood; Blood Coagulation Tests; methods; Child; Child, Preschool; Factor VIII; immunology; Female; Hemophilia A; blood; immunology; Humans; Male; Middle Aged; Sensitivity and Specificity; Young Adult
- From: Acta Academiae Medicinae Sinicae 2009;31(5):551-554
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the sensitivity and practicability of modified Bethesda assay and Nijmegen assay in detecting factor VIII (FVIII) inhibitor.
METHODSModified Bethesda assay and Nijmegen assay were used to screen FVIII inhibitors in 237 patients with hemophilia A. The buffer plus universal coagulation reference plasma (UCRP) was used to establish a standard curve for FVIII: C assay in modified Bethesda method, instead of Nijmegen plasma plus FVIII deficiency plasma in Nijmegen method. The cutoff value for positive FVIII inhibitors is > or = 0.6 BU/ml.
RESULTSThe positive rate of FVIII inhibitors was 5.5% (n = 13) when using modified Bethesda assay and was 8.4% (n = 20) when using Nijmegen assay (P > 0.05).
CONCLUSIONModified standard Bethesda assay is a convenient and feasible method for detecting FVIII inhibitors.
