Histone lysine methyltransferase Setd7 enhances Ngn 1 gene expression.
- Author:
Jin-Po DAI
1
;
Ye ZHANG
;
Yu-Fei SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Basic Helix-Loop-Helix Transcription Factors; genetics; metabolism; Gene Expression Regulation; Genetic Vectors; HEK293 Cells; Histone-Lysine N-Methyltransferase; genetics; metabolism; Humans; Mice; Nerve Tissue Proteins; genetics; metabolism; Protein Methyltransferases; genetics; metabolism; RNA, Messenger; genetics; Transfection
- From: Acta Academiae Medicinae Sinicae 2009;31(6):692-695
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct the eukaryotic expression plasmid of mouse histone lysine methyltransferase Setd7 and detect its effect on neuron development.
METHODSThe clone of mouse Setd7 was obtained and inserted into the eukaryotic expression vector pCMV-3tag-6-Flag. The plasmid was transfected into HEK 293T and identified by Western blot. Real-time PCR was used to detect the effect of Setd7 on the neuron differentiation marker gene Ngn 1 mRNA expression. Dual luciferase reporter system was used to detect the effect of Setd7 on Ngn 1 mRNA expression. Real-time PCR was used to detect the effect of Setd 7 siRNA plasmid on Ngn 1 mRNA expression.
RESULTSAn eukaryotic expression plasmid of Setd 7 was successfully constructed. Setd7 induced Ngn 1 mRNA expression and increased Ngn 1 promoter activity. Also, the knockdown of Setd 7 inhibited Ngn 1 mRNA expression.
CONCLUSIONHistone lysine methyltransferase Setd7 can enhance neuron differentiation marker gene Ngn 1 transcription.
