Role of Ezh2 in the all-trans retinoic acid induced P19 neural differentiation.
- Author:
Cui-ting GE
1
;
Ye ZHANG
;
Yu-fei SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Basic Helix-Loop-Helix Transcription Factors; genetics; metabolism; Cell Differentiation; drug effects; physiology; Enhancer of Zeste Homolog 2 Protein; Histone-Lysine N-Methyltransferase; genetics; metabolism; Histones; metabolism; Mice; Nerve Tissue Proteins; genetics; metabolism; Neurons; cytology; drug effects; metabolism; Polycomb Repressive Complex 2; Tretinoin; pharmacology; Tumor Cells, Cultured
- From: Acta Academiae Medicinae Sinicae 2009;31(6):707-711
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the role of Ezh2 in the all-trans retinoic acid RA induced P19 neural differentiation.
METHODSThe expression of Ngn1 in the RA induced P19 cells was detected at the mRNA and protein levels using real time RT-PCR and Western blot assays. The binding of Ezh2 and H3K27me3 on the Ngn 1 promoter was analyzed using chromatin immunoprecipitation assay.
RESULTIn the RA induced P19 cells, the recruitment of Ezh2 and its methylated substrate H3K27me3 on the promoter of Ngn 1 gene elevated in the first 2 days, and then declined rapidly, followed by the initiation of neuronal differentiation.
CONCLUSIONSEzh2 produces a repressive histone mark H3K27me3 in the early stage of RA induced P12 cells. By avoiding the premature expression of Ngn1 gene, Ezh2 can ensure the normal differentiation of P19 cells.
