Heat shock induced the expression of major histocompatibility complex class transactivator and human leukocyte antigen-DR in Jurkat cells.
- Author:
Li YAN
1
;
Mo-bin CHENG
;
Ye ZHANG
;
Yu-fei SHEN
Author Information
- Publication Type:Journal Article
- MeSH: HLA-DR Antigens; metabolism; Heat-Shock Response; physiology; Humans; Jurkat Cells; Nuclear Proteins; genetics; metabolism; RNA, Messenger; genetics; Trans-Activators; genetics; metabolism
- From: Acta Academiae Medicinae Sinicae 2009;31(6):746-750
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of a non-lethal heat shock, in comparison with the treatment of interferon-gamma (IFN gamma), on the expression of major histocompatibility complex transactivator (CTA) and its downstream target gene of the human leukocyte antigens (HLA)-DR in Jurkat cells.
METHODSThe changes of CTA mRNA in Jurkat cells before and after the treatment of heat shock or IFN gamma were detected using real time RT-PCR. The changes of CTA protein were detected with Western blot. The expression of HLA-DR was detected with flow cytometry. : CTA mRNA and protein were induced in Jurkat cells under heat shock, but not with IFN-gamma. The expression of HLA-DR gene significantly increased after recovery (P<0.01).
CONCLUSIONThe expressions of CTA and HLA-DR in Jurkat cells remarkably increase after heat shock, indicating that heat shock may help reconstruct relevant genes in cells with immunologic gene deficiencies.
