Effect of PIH1D1 on the degradation of its binding protein SNF5.
- Author:
Niu ZHAI
1
;
Ye ZHANG
;
Yu-fei SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis Regulatory Proteins; genetics; metabolism; Chromosomal Proteins, Non-Histone; metabolism; DNA-Binding Proteins; metabolism; Genetic Vectors; HEK293 Cells; Humans; Plasmids; genetics; Proteasome Endopeptidase Complex; metabolism; SMARCB1 Protein; Transcription Factors; metabolism; Transfection
- From: Acta Academiae Medicinae Sinicae 2009;31(6):756-759
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of PIH1D1 on its binding protein SNF5, a core subunit of the SWI/SNF chromatin remodeling complex.
METHODThe degradation pathway of SNF5 was identified with protein synthesis inhibitor cycloheximide (CHX) and a potent proteasome inhibitor MG132, and then the PIH1D1 eukaryotic expression plasmid was transfected to explore its effect on the stability of SNF5.
RESULTSHEK293T cells were effectively treated with CHX (optimal concentration: 400 microg/ml) and MG132 (optimal concentration: 20 mmol/L). The degradation of SNF5 was mediated by the proteasome pathway. PIH1D1 regulated the protein level of SNF5 by attenuating its proteasome degradation.
CONCLUSIONPIH1D1 may stabilize SNF5 by attenuating its proteasome degradation pathway.
