Effects of Rb1 and Rg1 on the expression of Bcl-2, Bax in apoptosis of HK-2 cells induced by the serum of kidney ischemia/reperfusion.
- Author:
Min-Xia ZHU
1
;
Bing RAN
;
Zhi-Qiang FENG
;
Qiang-Wen PAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Cell Line; Female; Ginsenosides; pharmacology; Ischemia; blood; Kidney; blood supply; Kidney Tubules, Proximal; cytology; Male; Proto-Oncogene Proteins c-bcl-2; genetics; metabolism; Rabbits; Reperfusion Injury; blood; Serum; physiology; bcl-2-Associated X Protein; genetics; metabolism
- From: Chinese Journal of Applied Physiology 2009;25(4):496-499
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo explore effects of Ginsenosides (Rb1, Rg1) on the expression of Bcl-2, Bax in the serum of kidney ischemia/reperfusion inducing apoptosis of HK-2 cells.
METHODSThe serum of rabbits with renal ischemia/reperfusion (SIR) and the control serum of rabbits (SC) were acquired and cultured with HK-2 cells. Detected apoptosis with TUNEL assay. The experiment was designed as: control group,ischemia/ reperfusion group, Rb1 blocking group and Rg1 blocking group. To detect the expression of Bcl-2, Bax with immunocytochemistry after 24 hours' cultured.
RESULTSThe expression of Bax in Rb1 blocking group and Rg1 blocking group were significantly decreased (P < 0.01), the ratios of Bcl-2/Bax were increased as compared with ischemia/reperfusion group.
CONCLUSIONRb1 and Rg1 have protective effects on apoptosis of HK-2 cells induced by serum of kidney ischemia/reperfusion.
