Effect of curcumine on the nuclear pathway of JNK during hippocampal ischemia/reperfusion injury in SHR.
- Author:
Ke-Ping YE
1
;
Chun-Ru CHEN
;
Jin-Wei ZHENG
;
Hong CAO
;
Bin JI
;
Rui ZHOU
;
Zhi-Yan MENG
;
Jun LI
;
Qing-Quan LIAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Brain Ischemia; metabolism; pathology; CA1 Region, Hippocampal; drug effects; metabolism; Curcumin; pharmacology; JNK Mitogen-Activated Protein Kinases; metabolism; Male; Neurons; drug effects; metabolism; Proto-Oncogene Proteins c-fos; metabolism; Rats; Rats, Inbred SHR; Rats, Inbred WKY; Reperfusion Injury; metabolism; pathology
- From: Chinese Journal of Applied Physiology 2010;26(4):416-420
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the diversify of the nuclear pathway of c-Jun NH2-terminal kinases (JNK) during transient brain ischemia/reperfusion injury in hippocampal neuron apoptosis in spontaneously hypertensive rats (SHR) and to test whether the neuroprotection of curcumine on transient brain ischemia/reperfusion injury in SHR is related to the nuclear pathway of JNK.
METHODSMale Wistar-Kyoto (WKY) rats and SHR were randomly divided into five groups (n = 6): WKY sham group (W-Sham), WKY ischemia/reperfusion group (W-I/ R), SHR sham group (S-Sham), SHR ischemia/reperfusion group (S-I/R) and SHR curcumine (a chinese traditional medicine)100 mg/kg treatment group (S-Cur), which were sacrificed at 2 h, 6 h, 24 h, 3 d and 7 d after reperfusion. Global brain ischemic model was established by 4-VO method. The TdT-mediated dUTP nick end labeling (TUNEL) method was used to detect the neuron apoptosis in hippocampal CA1 region. The immunohistochemical method was applied to investigate the expressions of c-jun and c-fos in hippocampal CA1 region.
RESULTSThe expressions of apoptosis and c-jun and c-fos in CA1 region in S-Sham group, W-I/R group and S-I/R group were more than those in W-Sham group (P < 0.05), were significantly increased in S-I/R group than those in W-I/R group (P < 0.05), and were significantly decreased in S-Cur group than those in S-I/R group (P < 0.05).
CONCLUSIONNeuronal apoptosis and the expressions of c-jun and c-fos are more in SHR hippocampal. Global brain ischemia/reperfusion injury induces more expressions of apoptosis in hippocampal neuron in SHR, and the more expressions of c-jun and c-fos may participate in that process. The neuroprotection of curcumine in SHR is related to c-jun and c-fos.