The effect of Crocin against hypoxia damage of myocardial cell and its mechanism.
- Author:
Yang WU
1
;
Rui-Rong PAN
;
Peng GENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carotenoids; pharmacology; Cell Hypoxia; drug effects; Cells, Cultured; Homeodomain Proteins; metabolism; Hypoxia-Inducible Factor 1, alpha Subunit; metabolism; Hypoxia-Inducible Factor-Proline Dioxygenases; metabolism; Myocytes, Cardiac; drug effects; metabolism; Nitric Oxide Synthase Type II; metabolism; Procollagen-Proline Dioxygenase; metabolism; Rats; Rats, Sprague-Dawley; Vascular Endothelial Growth Factor A; metabolism
- From: Chinese Journal of Applied Physiology 2010;26(4):453-457
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protective effect of Crocin against hypoxia damage of cardiac myocytes of neonatal rats and the regulation of HIF-1 and prolyhydroxylase (PHDs).
METHODSA model of CoCl2 simulated hypoxia damage was established in primary cultural myocardial cell. Expression levels of HIF-1alpha, VEGF, iNOS, as well as PHD1, 2, 3 protein in myocardial cells were detected by Western blot.
RESULTSCompared with CoCl2 group, the viability of myocardial cell was significantly increased after treated 24 h at 10(-5)mol/L Crocin (P < 0.01), HIF-1alpha, VEGF and iNOS were expressed higher than those in Crocin + CoCl2 group (P < 0.01), the expression of PHD2 was significantly increased (P < 0.01), while the expression of PHD3 was remarkably reduced in Crocin + CoCl2 Group (P < 0.01).
CONCLUSIONCrocin has better protective effect on hypoxic damage of myocardial cell. The mechanisms of protective effect of Crocin may be related to the activation of HIF-1-mediated pathway of the hypoxia response. PHDs may be involved in the pathophysiology regulated process of myocardial cells.
