Protective effect of limb ischemic preconditioning against liver ischemia/reperfusion injury in the rat.
- Author:
Hou-Wen JIANG
1
;
Chuang CHEN
;
Li-Jun HAO
Author Information
- Publication Type:Journal Article
- MeSH: Alanine Transaminase; blood; Animals; Aspartate Aminotransferases; blood; Ischemic Preconditioning; Liver; blood supply; metabolism; Male; Rats; Rats, Sprague-Dawley; Reperfusion Injury; prevention & control
- From: Chinese Journal of Applied Physiology 2010;26(4):502-504
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the protective effect of limb ischemic preconditioning against liver ischemia/reperfusion injury in the rat.
METHODSRats were randomly divided into four groups (n = 8): (1) Sham group (S group), rats without ischemic preconditioning (IPC), (2) Ischemia/reperfusion (I/R) without IP (I/R group); (3) Rats with 5 min IPC (IPC group); (4) Rats with lower limbs IPC and repeated three times (remote ischemic preconditioning, RPC group); The rats were subjected to 60-min sustained liver ischemia followed by 180-min reperfusion except S group. All ischemia rats were only subjected to 70% liver ischemia. Finally, blood and liver samples were obtained to determine the activity of ALT and AST, liver wet/dry weight (W/D), PMN counts and pathology.
RESULTSAll IPC group and RPC group had obviously lower levels of ALT, AST, W/D, PMN counts than that of the I/R group (P < 0.01).
CONCLUSIONThe limb ischemic preconditioning has a protective effects against liver ischemia/reperfusion injury in the rat, possibly are due to suppression of liver inflammatory reaction, improvement of liver microcirculation.
