The effect of high frequency stimulation of epileptic foci on the release of glutamate and gamma-aminobutyric acid in hippocampus of the kainic acid-kindled rats.
- Author:
Wan-Hui LIN
1
;
Hua-Pin HUANG
;
Ming-Xing LIN
;
Sheng-Gen CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Electric Stimulation; methods; Epilepsy; chemically induced; therapy; Glutamic Acid; secretion; Hippocampus; metabolism; Kainic Acid; Kindling, Neurologic; drug effects; Male; Rats; Rats, Sprague-Dawley; gamma-Aminobutyric Acid; secretion
- From: Chinese Journal of Applied Physiology 2011;27(1):88-92
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the dynamics of hippocampal release of glutamate (Glu) and gamma-aminobutyric acid (GABA) in epilepsy (TLE) after administration with high frequency stimulation (HFS).
METHODSThe SD were divided into four groups (n =10): (1) Control group (KB) the rats were injected intraperitoneally with saline 0.9%. (2) Kainic acid (KA) group: the rats were injected with KA. (3) Pseudo-deep brain stimulation (DBS) group: the KA-induced rats were implanted with rheophores alone. (4) DBS group: KA induced-rats with DBS in hippocampal epileptic foci. We then collected hippocampal extracellular fluid by microdialysis and the levels of Glu and GABA were measured by high-performance liquid chromatography (HPLC) and fluorescence detection.
RESULTSThere was no difference in the baseline of Glu and GABA in the four groups. In contrast, a significant increase in the content of Glu and GABA was shown in the three periods of KA-kindled seizures. Electrical stimulation of hippocampus resulted in a decrease of hippocampal Glu contents, while there was no change in GABA contents. Additionally, HFS of hippocampus normalized the Glu/GABA ratio in the chronic period of seizures.
CONCLUSIONThe high frequency stimulation of epileptic foci may protect against seizures by modulating the extracellular release of hippocampal Glu.
