Molecular mechanisms involved in the treatment of inflammatory bowel disease by Pulsatilla decoction.
- Author:
Shu-Wen LU
1
;
Hong-Ju LIU
;
Wei ZHAO
;
Li LI
;
Huan-Huan DUN
;
Chao LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Drugs, Chinese Herbal; therapeutic use; Inflammatory Bowel Diseases; drug therapy; Male; Phytotherapy; Pulsatilla; chemistry; Rats; Rats, Wistar; Signal Transduction; drug effects; Smad3 Protein; metabolism; Smad7 Protein; metabolism; Transforming Growth Factor beta1; metabolism
- From: Chinese Journal of Applied Physiology 2011;27(1):106-109
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate molecular mechanisms underlying in the treatment of inflammatory bowel disease by Pulsatilla decoction.
METHODSWistar male rats were randomly divided into control group, model group, model + positive control group (mesalazine), traditional Chinese medicine treatment group, in addition, the Chinese medical treatment group was divided into middle and high dose group ( n = 8). Intragastric administration was used in the positive control group and traditional Chinese medicine treatment group. The expression of Smad7 and p-Smad3 in the colons were detected by immunohistochemistry and Western blot.
RESULTSCompared with the model group, positive medicine and traditional Chinese medicine group, especially high-dose group, could effectively inhibit the expression of Smad7, while enhancing the p-Smad3 expression.
CONCLUSIONThe activation of TGF-beta1/Smad3 signaling pathway may be the molecular mechanism underlying in the anti-inflammatory effect of inflammatory bowel disease by Pulsatilla decoction.
