Effect of hypoxia on activation of the peripheral blood T lymphocyte in rats.
- Author:
Yun-Mei TIAN
1
;
Hong-Jing NIE
;
Jia-Ying LIU
;
Yan-Kun ZHANG
;
Dong-Xiang ZHANG
;
Hai WANG
Author Information
- Publication Type:Journal Article
- MeSH: Altitude; Altitude Sickness; physiopathology; Animals; CD4-Positive T-Lymphocytes; physiology; CD8-Positive T-Lymphocytes; physiology; Hypoxia; immunology; physiopathology; Lymphocyte Activation; physiology; Male; Rats; Rats, Wistar; T-Lymphocytes; physiology
- From: Chinese Journal of Applied Physiology 2011;27(2):145-148
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of hypoxia on the peripheral blood T lymphocyte subsets and co-stimulatory molecules in rats so as to provide the basis for studying the intervention measure.
METHODSBefore hypoxia and during hypoxia at 8 000 m for 8 h, 3 d, 6 d and 10 d the change of peripheral blood T lymphocyte subsets and co-stimulatory molecules in rats were detected by flowcytometer with three-color immunofluorescence label.
RESULTSRats were exposed to hypoxia at 8 000 m for 8 hours, and CD3+, CD8+, CD8+ CD28- lymphocyte percentages were significantly decreased (P < 0.01) compared with that before hypoxia. After 3 days of hypoxia, besides aforesaid change, CD4+ CD28+ lymphocyte percentage also prominently decreased (P < 0.01) and CD4+ CD28- prominently increased (P < 0.01). After 6 and 10 days of hypoxia, CD3+, CD4+ lymphocyte percentages were further decreased, while CD8+ CD28+ lymphocyte percentage significantly increased (P < 0.01).
CONCLUSIONAfter exposed to hypoxia at 8 000 m for 8 hours and 3 days, activation of CD8+ and CD4+ T lymphocyte was prominently decreased, while with the prolong of exposed time activation of CD8+ T lymphocyte was significantly increased.
