Irradiation induced epithelial-mesenchymal transition in nasopharyngeal carcinoma in vitro
10.3760/cma.j.issn.1673-0860.2013.08.011
- VernacularTitle:放射线照射诱导鼻咽癌细胞上皮-间质转化的作用研究
- Author:
Guo LI
1
;
Yong LIU
;
Zhong-Wu SU
;
Shu-Ling REN
;
Chao LIU
;
Yong-Quan TIAN
;
Yuan-Zheng QIU
Author Information
1. 410008长沙,中南大学湘雅医院耳鼻咽喉头颈外科,耳鼻咽喉重大疾病研究湖南省重点实验室
- Keywords:
Nasopharyngeal neoplasms;
Epithelial-mesenchymal transition;
Radiotherapy;
Radiation tolerance
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2013;48(8):662-667
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the irradiation induced epithelial-mesenchymal transition (EMT) in nasopharyngeal carcinoma (NPC) in vitro.Methods NPC CNE-2 cells with radioresistance (CNE-2-Rs) were established by exposure to gradiently increased dose of irradiation.CCK-8 cell viability kits,colony formation assay and fluorescence-activated cell sorting analysis were used to confirm the capacity of radioresistance of CNE-2-Rs cells.Invert microscope was used to monitor the morphological changes and western blot was applied to detect the expression of epithelial cell marker E-cadherin and mesenchymal cell marker Vimentin during the phase of CNE-2 exposure to irradiation.Results Irradiation exposure successfully induced the radioresistance of CNE-2 cells.After exposed to irradiation,the survival rate in CNE-2-Rs was higher than that in CNE-2 by CCK-8 assays.No significant difference of proliferation ability was observed between the CNE-2 and CNE-2-Rs pre-radiotherapy,but a higher proliferation ability in the CNE-2-Rs post-radiotherapy.By using the colony forming assay,the parameters of CNE-2 and CNE-2-Rs in multi-target single-hit and linear quadratic model were obtained and the data demonstrated that parameters mean lethal dose (D0),quasi-thres hold dose (Dq),surrival fraction in 2Qy (SF2) and mean inctivation dose (MID) value increased,α and α/β value decreased(P ＜0.05).At the same time,the CNE-2-Rs cells showed higher percentage of cells in S and G2 phase(P ＜ 0.05).In terms of biomorphology,CNE-2-Rs cells were more narrow,long strips or fusiform shapes,stretched out tentacles,and the contacts between them were loosened.When radiation dose accumulated to 24 Gy,an over-expression of Vimentin was observed in treated cells,while E-cadherin was down-regulated (P ＜ 0.01).Conclusions NPC cells present with typical morphorlogical and biomolecular changes of EMT during exposure to irradiation,indicating the potential critical roles of EMT in the malignant behavior of radioresistance in NPC.
