Inhibition of micro RNA-9 expression promotes UV-induced ROS damage in nasopharyngeal carcinoma cells
10.3760/cma.j.issn.1673-0860.2013.08.012
- VernacularTitle:负向调控鼻咽癌微小RNA-9表达促进紫外线介导的活性氧损伤
- Author:
Chao-Pan ZHENG
1
;
Ling HAN
;
Wei-Jian HOU
;
Yi-Hui WEN
;
Ran FU
;
Ren-Qiang MA
;
Wei-Ping WEN
Author Information
1. 518020,深圳市人民医院耳鼻咽喉科
- Keywords:
Nasopharyngeal neoplasms;
MicroRNAs;
Reactive oxygen species;
Ultraviolet rays
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2013;48(8):668-672
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of down-regulated miR-9 expression on ultraviolet rays (UV)-induced reactive oxygen species (ROS) damage in nasopharyngeal carcinoma (NPC) cells.Methods The NPC cells were transfected with inhibitors of miR-9 by lipofectamine to decrease the expression of miR-9,and the cells transfected with inhibitor control as the control.ROS levels following UV exposure were examined with DCF-DA method and the concentration of glutathione was analyzed via the benzoic acid method; DNA damage and apoptosis also were evaluated.Results There was significant difference in ROS levels between miR-9 expression-inhibited cells and control cells (26 895 ± 218 vs 15 765-±927,t =39.754,P ＜0.001),and also there were significant differences in DNA damage rates (28.0％ ± 10.0％ vs 23.6％ ±9.2％) and in apoptosis rates (8.0％ ±0.9％ vs 4.5％ ±0.8％) following UV exposure between two groups of cells.The miR-9 expression-inhibited cells showed lower level (1.87 ± 0.15) μmoL/L of glutathione compared with the control cells (9.85 ± 0.15) μmol/L (t =-48.832,P＜0.001).Conclusion Inhibition of miR-9 expression promoted UV-induced ROS damage in nasopharyngeal carcinoma cells.
