Expression of peripheral blood γδ T cells, Treg cells and cytokines IL-17 and TGF-β1 in patients with allergic rhinitis
10.3760/cma.j.issn.1673-0860.2013.07.005
- VernacularTitle:γδT细胞、调节性T细胞、白细胞介素17及转化生长因子β1在变应性鼻炎患者外周血中的表达
- Author:
Xue-Kun HUANG
1
;
Qin-Tai YANG
;
Yu-Lian CHEN
;
Fu-Cheng ZHANG
;
Ge-Hua ZHANG
Author Information
1. 中山大学附属第三医院耳鼻咽喉科
- Keywords:
Receptors,antigen,T-cell,gamma-delta;
T-lymphocytes,regulatory;
Interleukin-17;
Transforming growth factor beta 1;
Rhinitis,allergic,perennial;
Rhinitis,allergic,seasonal
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2013;48(7):544-548
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of peripheral blood γδ T cells/CD4 + CD25 + regulatory T cells(Treg) and cytokines interleukin 17 (IL-17) and transforming growth factor β1 (TGF-β1) in patients with allergic rhinitis.Methods From March 2012 to July 2012,32 patients with allergic rhinitis (AR group) and 20 healthy control subjects (control group) were collected.The expression of peripheral blood γδ T cells/Treg cells were measured by flow cytometry and the levels of IL-17 and TGF-β1 were evaluated by ELISA.SPSS 16.0 software was used to analyze the data.Results The percentages of γδT cells in AR group were (13.30 ±8.62)％,which was significantly higher (t =5.18,P ＜0.01) than those in control group (5.18 ± 1.86)％.The percentages of Treg cells in AR group were (1.75 ±0.56)％,which were significantly lower (t =7.46,P ＜ 0.01) than those in control group (4.76 ± 1.74) ％.The IL-17 levels in AR group were (668.55 ±45.15) pg/ml,which were also significantly higher (t =8.97,P ＜ 0.01) than those in control group (573.53 ± 17.42) pg/ml.The TGF-β1 levels in AR group were (0.34 ±0.04) pg/ml,which were also significantly lower (t =9.51,P ＜0.01) than those in control group(0.49 ± 0.06) pg/ml.There was a negative correlation between the percentages of γδ T cells and Treg cells (r =-0.561,P ＜0.01).There was a negative correlation between the percentages of γδ T cells and TGF-β1 levels (r =-0.622,P ＜ 0.01).A positive correlation was shown between the percentages of βδ T cells and IL-17 levels in AR (r =0.469,P ＜ 0.01).A positive correlation was shown between the percentages of Treg cells and TGF-β1 levels in AR (r =0.738,P ＜0.01).There was no correlation between IL-17 levels and the percentages of Treg cells or TGF-β1 levels (r value was-0.111,-0.196,all P ＞ 0.05).Conclusion There are imbalances of γδ T and Treg cells in peripheral blood of patients with allergic rhinitis.γδ T cells may be the main cell to produce IL-17,which may play an important role in allergic rhinitis.
