Effect of neonatal immunization with different dosage allergen on the immunity of mice when grown up
10.3760/cma.j.issn.1673-0860.2013.07.009
- VernacularTitle:不同剂量变应原免疫对新生小鼠成年后免疫状态的影响
- Author:
Jian-Jun CHEN
1
;
Jing-Hui WANG
;
Yang YUAN
;
Jun-Mei FU
;
Zi-Zhong YU
;
Yan-Jun WANG
;
Wei-Jia KONG
Author Information
1. 华中科技大学同济医学院附属协和医院耳鼻咽喉科
- Keywords:
Ovalbumin;
Allergens;
Hypersensitivity;
T-lymphocytes,helper-inducer;
Immunologic memory;
Cytokines;
Mice;
Animals,newborn
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2013;48(7):563-567
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of neonatal immunization with different dosage allergen on the immunity of mice when grown.Methods Fifty neonatal BALB/c mice were divided into 4 groups randomly and subcutaneous injected with different dosage of ovalbumin (OVA) on day 1,8 and 15 after born [NS group(10):injected with saline alone ; NS + AL group(10):injected with saline and AL(OH) 3 ; small dosage (SD) group (15):injected with 10 μg OVA and AL (OH) 3 ; large dosage (LD) group (15):injected with 1000 μg OVA and AL(OH)3].The mice were then challenged using caudal vein injection on 5 weeks old (NS group and NS + AL group were challenged with saline,SD group and LD group were challenged with 100 μg OVA).The blood was collected 1 week later to examine OVA specific IgE,IgG1 and IgG2a.Mononuclear cells were drawn from the spleen and cultured.Concentration of IL-4,IFN-r,IL-10 was examined in the cultural supernatant.Flow cytometry was used to test the expression of CD4 + IL-4 +,CD4 + IFN-γ+,CD4 + IL-10 + cells.Results It was found that concentration of OVA specific IgE (OVA-sIgE) in SD group (0.33 ± 0.18) was significantly higher than that of NS (0.07 ± 0.01) and NS + AL (0.09±0.04) group (t value was-3.46 and-3.21,all P＜0.01),and LD group (0.17 ±0.10) as well (t =2.58,P ＜0.05).The concentration of OVA-sIgE was higher in LD group than that of NS group (t=-2.53,P＜0.05),but similar with that of NS +AL group (t =-2.04,P ＞0.05).Both the concentration of OVA-sIgG1 and sIgG2a was higher in SD and LD group than that of NS and NS + AL group (all P ＜ 0.05).The concentration of IL-4,IFN-γ and IL-10 in the cultural supernatant of spleen mononuclear was all higher in SD group than that of NS and NS + AL group (all P ＜ 0.01).The ratio of IFN-γ/IL-4 was significantly lower in SD group than that of NS and NS + AL group (t value was 2.14,3.44,all P ＜ 0.05),while the same ratio was higher in LD group than that of NS and NS + AL group (t value was-2.14,-1.61,all P ＜0.05).Ratio of CD4+ IL-4+ cells was significant lower in LD group than that of SD group (P ＜ 0.05),while it was not different with that of NS and NS + AL group (P ＞ 0.05).Conclusion Neonatal immunization with low dosage OVA could generate a specific immunity with Th2 direction,while with large dosage OVA could generate a specific immunity with Th1 direction.
