Enhanced Expression of Aquaporin-9 in Rat Brain Edema Induced by Bacterial Lipopolysaccharides
10.1007/s11596-009-0203-4
- Author:
WANG HUAILI
1
,
2
;
JIN RUNMING
;
TIAN PEICHAO
;
ZHUO ZHIHONG
Author Information
1. Department of Pediatrics,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China
2. Department of Pediatrics,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China
- Keywords:
aquaporins;
aquaporin-9;
lipopolysaccharides;
infectious brain edema;
water channels
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2009;29(2):150-155
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the role of AQP9 in brain edema,the expression of AQP9 in an infectious rat brain edema model induced by the injection of lipopolysaccharide (LPS) was examined.Immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) analysis demonstrated that the expressions of AQP9 mRNA and protein at all observed intervals were significantly increased in LPS-treated animals in comparison with the control animals.Time-course analysis showed that the first signs of blood-brain barrier disruption and the increase of brain water content in LPS-treated animals were evident 6 h after LPS injection,with maximum value appearing at 12 h,which coincided with the expression profiles of AQP9 mRNA and protein in LPS-treated animals.The further correlation analysis revealed strong positive correlations among the brain water content,the disruption of the blood-brain barrier and the enhanced expressions of AQP9 mRNA and protein in LPS-treated animals.These results suggested that the regulation of AQP9 expression may play important roles in water movement and in brain metabolic homeostasis associated with the pathophysiology of brain edema induced by LPS injection.
