Endothelial Nitric Oxide Synthase Traffic Inducer in the Umbilical Vessels of the Patients with Pre-eclampsia
10.1007/s11596-009-0222-1
- Author:
XIANG WENPEI
1
;
CHEN HANPING
;
HU LIAN
;
XU XIAOYAN
Author Information
1. Center of Reproductive Medicine,Institute of Family Planning Research,Tongji Hospital,Tong]i Medical College,Huazhong University of Science and Technology University,Wuhan 430030,China
- Keywords:
OSTRIN;
pre-eclampsia;
umbilical vessel;
eNOS
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2009;29(2):243-245
- CountryChina
- Language:Chinese
-
Abstract:
The expression of endothelial nitric oxide synthase traffic inducer (NOSTRIN) was examined in the umbilical vessels of the patients with pre-eclampsia (PE) to explore its possible role in the pathogenesis of PE.The NOSTRIN mRNA in umbilical tissues was determined by RT-PCR.The eNOS activity in umbilical vessels was spectrophotometrically detected.NO2-/NO3-,the stable metabolic end products of NO,was measured by using nitrate reductase.RT-PCR showed that the expression level of NOSTRIN was significantly higher in women with PE than in the normal group (P<0.01).The activity of eNOS was significantly decreased in PE group [(12.83±3.61) U/mg] than in normal group [(21.72±3.83) U/mg] (P<0.01).The level of NO2-/NO3- in PE patients (27.53± 7.48) μmol/mg was significantly lower than that of normal group (54.27±9.53) μmol/mg (P<0.01).The significant negative correlation existed between the expression of NOSTRIN and the activity of eNOS in umbilical vessels of women with PE (r=-0.58,P<0.01).It was concluded that the level of NOSTRIN expression was increased in umbilical vessel of women with PE,indicating that it may be involved in the pathogenesis of PE.
