Stable Surface Expression of a Gene for Helicobacter pylori Toxic Porin Protein with pBAD Expression System
10.1007/s11596-009-0409-5
- Author:
PENG ZHIXIANG
1
;
WEI XI
;
LIN ZHENGMEI
Author Information
1. Department of Endodontics, Guanghua College of Stomatology, Sun Yat-Sen University, Guangzhou 510055, China
- Keywords:
hopE;
Helicobacter pylori;
porin;
pBAD expression system;
Escherichia coli;
lac op-eron-regulated plasmid
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2009;29(4):435-438
- CountryChina
- Language:Chinese
-
Abstract:
successive passages could express Hope protein, while only 1 from 5 E. coli colonies that contained lac operon-regulated plasmid encoding hopE gene could express HopE. Indi-rect immunofluorescence confirmed the expression of HopE on E. coli cell surface.