Inhibitory action of berberine on glucose absorption.
- Author:
Guo-yu PAN
1
;
Guang-ji WANG
;
Jian-guo SUN
;
Zhi-jiang HUANG
;
Xiao-chen ZHAO
;
Yi GU
;
Xiao-dong LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Berberine; pharmacokinetics; pharmacology; Caco-2 Cells; Glucose; pharmacokinetics; Glycoside Hydrolase Inhibitors; Humans; Hypoglycemic Agents; pharmacology; Intestinal Absorption; drug effects; Male; Maltose; metabolism; Rats; Rats, Sprague-Dawley; Sucrase; metabolism
- From: Acta Pharmaceutica Sinica 2003;38(12):911-914
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the absorption characteristics of berberine and its influence on glucose absorption.
METHODSRat recirculating perfusion model was used to study berberine absorption characteristics and Caco-2 cell model was used to explore the influence of berberine on disaccharidase, using HPLC to assay the appearance of glucose to indicate enzyme activities.
RESULTSBerberine was found to be hardly absorbed in the intestine (less than 5% in 2.5 h). However, sucrase and maltase activities were found to be inhibited by berberine, its ID50 to sucrase is 1.830 mg.L-1, and showed no dose dependent influence on maltase activity. Berberine also showed influence on glucose absorption. However, this effect is not significant.
CONCLUSIONBerberine may act as an alpha-glucosidase inhibitor, which is its main mechanism in diabetes treatment.
