Analysis of impurity in caderofloxacin.
- Author:
Ren-ling MA
1
;
Hong-hua ZHOU
;
Rong LIU
;
Er-hua WANG
;
Long-sheng SHENG
Author Information
- Publication Type:Journal Article
- MeSH: Anti-Infective Agents; chemistry; Carboxylic Acids; analysis; chemistry; Chromatography, High Pressure Liquid; methods; Drug Contamination; Fluoroquinolones; chemistry; Molecular Structure; Piperazines; chemistry; Quinolines; analysis; chemistry; Spectrometry, Mass, Electrospray Ionization
- From: Acta Pharmaceutica Sinica 2003;38(12):950-952
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo analyse the main impurity of caderofloxacin.
METHODSThe impurity of caderofloxacin was analysed and determinated by RP-HPLC/ESI/MS with a Zorbax SB-C18 (150 mm x 4.6 mm ID, 5 microns) column. The mobile phase was acetonitrile-0.5% acetic acid solution (17:83). A compound was synthesized: 1-cyclopropyl-8-(difluoromethoxy)-6-fluoro-1, 4-dihydro-7-(1-piperazinyl)-4-oxo-3-quinoline carboxylic acid (DMCA). Its HPLC chromatogram, UV and MS spectrum were compared with those of the impurity in caderofloxacin.
RESULTSThe molecular weight of the impurity was 14 less than that of caderofloxacin. It means the impurity was a CH2-group less than caderoflixacin. The tR, UV and MS of DMCA were the same as those of the impurity in caderofloxacin.
CONCLUSIONBased on the tR (HPLC), UV and MS, the impurity of caderofloxacin is confirmed as DMCA.
