Molecular mechanism of granulocytic differentiation of human promyelocytic leukemia HL-60 cells induced by all-trans retinoic acid.
- Author:
Jin WANG
1
;
Chi-hung TZENG
;
Ming-hui HUANG
;
Hong-xun FANG
;
Pei-gen XIAO
;
Rui HAN
;
Meng-su YANG
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Cell Cycle; Cell Differentiation; Gene Expression Profiling; Granulocytes; drug effects; pathology; HL-60 Cells; Humans; Oligonucleotide Array Sequence Analysis; Tretinoin; pharmacology
- From: Acta Pharmaceutica Sinica 2004;39(1):22-28
- CountryChina
- Language:English
-
Abstract:
AIMTo elucidate the molecular mechanism of granulocytic differentiation of human promyelocytic leukemia HL-60 cells induced by all-trans-retinoic acid (ATRA).
METHODSFlow cytometry was used to determine the cell cycle changes of HL-60 cells upon ATRA treatment. Gene expression profiles of HL-60 cells induced by 1 mumol.L-1 ATRA were obtained by using cDNA microarrays containing 9,984 genes and expressed sequence tags (ESTs).
RESULTSCell cycle analysis showed that 48%-73% of cells were arrested at G1/G0 phase upon ATRA treatment; cDNA microarray results demonstrated that the expression of genes encoding adhesion molecules, tissue remodeling proteins, transporters and ribosomal proteins were up-regulated in ATRA treated of HL-60 cells. Several genes involved in oxidase activation pathway were also differentially expressed.
CONCLUSIONATRA treatment induced growth arrest and differentiation in HL-60 cells, which is associated with regulation of the oxidase activation pathway and the expression of tissue remodeling proteins.
