Effects of (-), (+)-7-hydroxy-clausenamide on synaptic transmission in rat dentate gyrus in vivo.
- Author:
Chuan-jiang ZHU
1
;
Jun-tian ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Action Potentials; drug effects; Animals; Dentate Gyrus; drug effects; physiology; Drugs, Chinese Herbal; isolation & purification; pharmacology; Excitatory Postsynaptic Potentials; drug effects; Injections, Intraventricular; Lactams; isolation & purification; pharmacology; Lignans; isolation & purification; pharmacology; Male; Plants, Medicinal; chemistry; Rats; Rats, Sprague-Dawley; Rutaceae; chemistry; Stereoisomerism; Synaptic Transmission; drug effects
- From: Acta Pharmaceutica Sinica 2004;39(1):34-36
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate effects of (-), (+)-7-hydroxy-clausenamide (7-OH-Clau) on basal synaptic transmission in the dentate gyrus of anesthetized rats.
METHODSExtracellular recording of the population spike (PS) of hippocampal dentate gyrus following application of low-frequency stimulation (1/30 Hz, 1.0 mA).
RESULTSFifteen, thirty and sixty minutes after intracerebroventricular (icv) administration of an estimated final brain concentration of 2 x 10(-6) mol.L-1, (-)-7-OH-Clau caused over 30% increase relative to basal PS amplitude before administration, and 27%-41% increase relative to vehicle control (P < 0.05), while (+)-7-OH-Clau exhibited 18%-25% and 11%-20% decrease in the PS amplitude when compared with basal PS amplitude and vehicle control (P < 0.05), respectively.
CONCLUSIONThe basal synaptic transmission in the dentate gyrus of the rat hippocampus can be potentiated by (-)-7-OH-Clau and attenuated by (+)-7-OH-Clau, indicating that there was a stereoselective difference between the two enantiomers in the modulation of synaptic responses and plasticity.
