Simultaneous determination of the major isosteroidal alkaloids and their glucosides in the bulbs of Fritillaria by high performance liquid chromatography coupled with evaporative light scattering detection.
- Author:
Ping LI
1
;
Ling-jie ZENG
;
Song-lin LI
;
Zhi-ming BI
;
Ge LIN
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; analysis; Cevanes; analysis; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; Fritillaria; chemistry; Glucosides; analysis; Plants, Medicinal; chemistry
- From: Acta Pharmaceutica Sinica 2004;39(1):56-59
- CountryChina
- Language:English
-
Abstract:
AIMTo establish an HPLC-ELSD method for the simultaneous determination of five major bioactive isosteroidal alkaloids and gluco-alkaloids in the bulbs of Fritillaria namely peimissine, imperialine, sinpeinine A, imperialine-3 beta-glucoside and yibeinoside A.
METHODSA Nova-Pak C18 column (150 mm x 3.9 mm ID) was used. The chromatography was carried out with a linear gradient programming. The mobile phase was acetonitrile-water (containing 0.1% diethylamine) and the flow rate was 1.0 mL.min-1.
RESULTSThe linear range of peimissine was 13.1-288.2 mg.L-1 (r2 = 0.9975), imperialine-3 beta-glucoside 7.7-169.4 mg.L-1 (r2 = 0.9993), yibeinoside A 7.3-160.6 mg.L-1 (r2 = 0.9997), imperialine 16.5-363.0 mg.L-1 (r2 = 0.9992), sinpeinine A 8.7-191.4 mg.L-1 (r2 = 0.9942).
CONCLUSIONThe method is accurate with overall intra- and inter-day variation less than 5% and recovery more than 95%. The method was successfully applied to analyze five major bioactive alkaloids and gluco-alkaloids in three Fritillaria bulbs.
