Effects of 9-cis-retinoic acid on cell cycle and expression of cyclin D1 and cdk4 in lung cancer cells.
- Author:
Gui-jie REN
1
;
Zhi-fang LIU
;
Guo-qiang HU
;
Xiao-yan HU
;
Ke-li TIAN
;
Xue-yan YU
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; metabolism; pathology; Antineoplastic Agents; pharmacology; Carcinoma, Squamous Cell; metabolism; pathology; Cell Division; drug effects; Cell Line, Tumor; Cyclin D1; biosynthesis; Cyclin-Dependent Kinase 4; Cyclin-Dependent Kinases; biosynthesis; G1 Phase; drug effects; Humans; Lung Neoplasms; metabolism; pathology; Proto-Oncogene Proteins; Resting Phase, Cell Cycle; drug effects; S Phase; drug effects; Tretinoin; pharmacology
- From: Acta Pharmaceutica Sinica 2004;39(2):97-100
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the effects of 9-cis-retinoic acid (9-cis-RA) on cell cycle and expression of cyclin D1 and cdk4 in lung cancer cells.
METHODS9-cis-RA (1 x 10(-6) mol.L-1) was used to treat lung cancer cells for 24 h; Flow cytometry (FCM) was used to detect the percent of G0/G1 phase and S phase cells of three groups including blank control, DMSO control and 9-cis-RA groups; RT-PCR was used to analyze the expression changes of cyclin D1 and cdk4 before and after treatment with 9-cis-RA in lung cancer cells.
RESULTSThe percent of G0/G1 phase cells of 9-cis-RA groups was significantly higher than that of the control groups (P < 0.01 or P < 0.05) and the percent of S phase cells of 9-cis-RA groups was lower than that of the control groups (P < 0.01 or P < 0.05); the expression of cyclin D1 of PG, SPC-A1 and L78 cells was decreased (P < 0.01) and the expression of cdk4 of PG, A549 and L78 cells was also decreased (P < 0.01) after treatment with 9-cis-RA.
CONCLUSIONMost of the proliferation and the expression of cyclin D1 and cdk4 of PG, A549, SPC-A1 and L78 were inhibited by 9-cis-RA.
