Activation of Ca(2+)-activated K+ channels by oxyphenamone in rabbit mesenteric vascular smooth muscle cells.
- Author:
An-long LI
1
;
Zhong-wu LIU
;
Li-xia ZHU
;
De-chang ZHANG
;
Yi-xin YE
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cardiotonic Agents; pharmacology; Mesenteric Arteries; cytology; Muscle, Smooth, Vascular; cytology; Myocytes, Smooth Muscle; physiology; Organic Chemicals; Patch-Clamp Techniques; Potassium Channels, Calcium-Activated; drug effects; Rabbits
- From: Acta Pharmaceutica Sinica 2004;39(2):101-104
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the effects of oxyphenamone (Oxy) on activation of Ca(2+)-activated K+ channels in rabbit mesenteric vascular smooth muscle cells.
METHODSTo measure the effect of Oxy on the Ca(2+)-activated K+ channel (BK (Ca) channel) activity in rabbit mesenteric vascular smooth muscle cells by using whole cell patch clamp techniques.
RESULTSOxy reversibly increase BK (Ca) channel activity in rabbit mesenteric artery smooth muscle cells. Application of Oxy (0.1 mumol.L-1) to the perfusion solution caused significant increase in outward currents and its effect was completely abolished by washout; The outward currents K+ was inhibited by TEA (7.5 mmol.L-1); Oxy activated the BK (Ca) channel in a dose-dependent manner (0.01-10 mumol.L-1).
CONCLUSIONOxy directly increase the activity of BK (Ca) channel activity in rabbit mesenteric vascular smooth muscle cells in dose-dependent manner.
