Determination of loratadine in human plasma by HPLC with fluorescence detector and study on its bioavailability.
- Author:
Xiao-jie XU
1
;
Er-xin SHANG
;
Fu-rong QIU
;
Guo-guang MAO
;
Bing-ren XIANG
Author Information
- Publication Type:Journal Article
- MeSH: Area Under Curve; Biological Availability; Chromatography, High Pressure Liquid; methods; Fluorescence; Histamine H1 Antagonists, Non-Sedating; blood; pharmacokinetics; Humans; Loratadine; blood; pharmacokinetics; Male
- From: Acta Pharmaceutica Sinica 2004;39(2):123-126
- CountryChina
- Language:English
-
Abstract:
AIMTo establish an HPLC-fluorescence method for determination of loratadine in human plasma and evaluate its relative bioavailability.
METHODSAn Alltech-C18 column and a mobile phase of acetonitrile-water-glacial acetic acid-triethylamine (90:100:6:0.15) were used. The fluorescence detector was set at Ex 274 nm, Em 450 nm. The flow rate was 1 mL.min-1.
RESULTSThe calibration curve was linear over a concentration range of 0.2-30 micrograms.L-1. The limit of quantification was 0.2 microgram.L-1. The average method recoveries varied from 96% to 98%. The results showed AUC, Tmax, Cmax and T1/2 beta between the testing tablets, testing capsules and reference tablets had no significant difference (P > 0.05). Relative bioavailabilities were 107% +/- 17% and 100% +/- 14% respectively.
CONCLUSIONThe three formulations were bioequivalent.
