Study on the enantiomer separation of cetirizine dihydrochloride using proteinate- and amylose-based chiral stationary phase.
- Author:
Zhe-feng ZHANG
1
;
Geng-liang YANG
;
Gui-jian LIANG
;
Yu ZHOU
;
Yi CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Amylose; analogs & derivatives; Cetirizine; chemistry; isolation & purification; Chromatography, High Pressure Liquid; Histamine H1 Antagonists, Non-Sedating; chemistry; isolation & purification; Molecular Structure; Orosomucoid; Stereoisomerism
- From: Acta Pharmaceutica Sinica 2004;39(3):204-207
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the chromatographic behavior of cetirizine dihydrochloride on the proteinate- and amylose- based chiral stationary phases so as to optimizate the chromatographic condition of its enantiomers separation.
METHODSWhen using amylose-based, alpha1-acid glycoprotein and ovomucoid protein chiral stationary phase, the mobile phase was hexane-isopropyl alcohol-alcohol-trifluoroacetic acid (430:45:25:1), acetonitrile-10 mmol x L(-1) phosphate buffer solution (adjusted to pH 7.0 with sodium hydroxide) (4:96) and acetonitrile-20 mmol x L(-1) KH, PO4 solution (adjusted to pH 7.0 with triethylamine) (12.7:87.3), respectively. The temperature of proteinate column was 25 degrees C. The detective wavelength was 230 nm.
RESULTSThe two enantiomers could be separated on the two kinds of chiral stationary phases without derivatization and the resolution was above 2.0. The methods developed on the two kinds of chiral stationary phases are accurate, sensitive and specific.
CONCLUSIONBoth the proteinate- and amylose-based chiral stationary phases can be used to separate the enantiomers of cetirizine.
