Effects of fungal elicitor on inophyllums production in suspension cultured cells of Calophyllum inophyllum L.
- Author:
Huan-liang LUO
1
;
Yong GUO
;
Tang-bing CUI
;
Jian-guo DAI
;
Jun-song ZHANG
;
Bai-qiu XU
Author Information
- Publication Type:Journal Article
- MeSH: Calophyllum; cytology; metabolism; Cell Division; Cells, Cultured; Coumarins; metabolism; Culture Media; Mitosporic Fungi; physiology
- From: Acta Pharmaceutica Sinica 2004;39(4):305-308
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the effects of fungal elicitors on inophyllums production in suspension cultured cell of Calophyllum inophyllum Linn.
METHODSThe pathogen of leaf spot disease of C. inophyllum L. was isolated and prepared as fungal elicitor. The fungal elicitor was added to the medium with different concentrations and culture period. Their effects on biomass and inophyllums content of the suspension of cultured cells were studied.
RESULTSThe optimum effects of S-I fungal elicitor concentrations on inophyllums content was 60 mg GE x L(-1). Adding the fungi elicitor into the cell suspension culture system at stationary phase (being cultured for 18 days) resulted in a highest inophyllum content of 59.174 mg x L(-1) at the 3rd day with 27% higher than control. Fungal elicitor treatment promoted the inophyllums accumulation in medium.
CONCLUSIONAdding the Stagonospora curtisii (Berk.) Sacc. to the medium was effective approaches to enhance inophyllums yield in the suspension of C. inophyllum L culture cell.
