5-HT2C receptor subtype modulate production of secretory beta-amyloid protein precursor in incubated rat hippocampal slices.
- Author:
Gui-rong ZHANG
1
;
Deng-lian GAO
;
Long-guang CAO
;
Ming YIN
Author Information
- Publication Type:Journal Article
- MeSH: Amyloid beta-Protein Precursor; secretion; Animals; Hippocampus; metabolism; In Vitro Techniques; Male; Peptide Fragments; secretion; Rats; Rats, Sprague-Dawley; Receptor, Serotonin, 5-HT2C; Serotonin; pharmacology; Serotonin 5-HT2 Receptor Agonists; Serotonin 5-HT2 Receptor Antagonists
- From: Acta Pharmaceutica Sinica 2004;39(5):321-324
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo determine whether serotonin, a major neurotransmitter in brain, can modulate the production of secretory beta-amyloid protein precursor (sAPP) by activation of serotonin 5-HT2C receptor.
METHODSThe hippocampal slices of rats were incubated with various concentrations of serotonin, M-110, or L-107. sAPP released into the incubation medium were assayed by Western blot analysis assay with monoclonal antibody 22C11 for 2 h.
RESULTSVarious concentrations of serotonin (1.0 x 10(-2) - 1.0 x 10(3) micromol x L(-1)), M-110, a serotonin 5-HT2C agonist (1.5 x 10(-6) - 1.5 x 10(3) micromol x L(-1)), showed positive effect on the production of sAPP while L-107, a serotonin 5-HT2C antagonist (1.0 x 10(-9) - 1.0 x 10(3) micromol x L(-1)), showed negative effect on the production of sAPP over controls.
CONCLUSIONSerotonin modulates production of secretory amyloid beta-protein precursor through serotonin 5-HT2C receptor in incubated rat hippocampal slices.
