Ethanol extract of Phellinus merrillii protects against diethylnitrosamine- and 2-acetylaminofluorene-induced hepatocarcinogenesis in rats.

Chun-hung Yang; Heng-yuan Chang; Yi-chuan Chen; Chia-chen LU; Shyh-shyun Huang; Guan-jhong Huang; Hsin-chih Lai

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Ethanol extract of Phellinus merrillii protects against diethylnitrosamine- and 2-acetylaminofluorene-induced hepatocarcinogenesis in rats.

Author: Chun-Hung YANG ¹ ; Heng-Yuan CHANG ² ; Yi-Chuan CHEN ² ; Chia-Chen LU ¹ ; Shyh-Shyun HUANG ³ ; Guan-Jhong HUANG ⁴ ; Hsin-Chih LAI ⁵
Author Information

1. Graduate Institute of Biomedical Science, Division of Biotechnology, College of Medicine, Chang Gung University, Tao-Yuan, 333, Taiwan, Republic of China.
2. School of Post-Baccalaureate Chinese Medicine, Tzu Chi University, Hualien, Taiwan, 970, China.
3. School of Pharmacy, College of Pharmacy, China Medical University, Taichung, Taiwan, 404, China.
4. School of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, Taichung, Taiwan, 404, China. gjhuang@mail.cmu.edu.tw.
5. Graduate Institute of Biomedical Science, Division of Biotechnology, College of Medicine, Chang Gung University, Tao-Yuan, 333, Taiwan, Republic of China. hclai@mail.cgu.edu.tw.
Publication Type:Journal Article
Keywords: 2-acetylaminofluorene; Phellinus merrillii; chemoprevention; diethylnitrosamine; hepatocarcinogenesis
MeSH: 2-Acetylaminofluorene; Animals; Basidiomycota; chemistry; Carcinogenesis; chemically induced; Cytoprotection; drug effects; Diethylnitrosamine; Ethanol; chemistry; Liver Neoplasms, Experimental; chemically induced; prevention & control; Male; Plant Extracts; chemistry; pharmacology; Protective Agents; pharmacology; Rats; Rats, Sprague-Dawley
From: Chinese journal of integrative medicine 2017;23(2):117-124
CountryChina
Language:English
Abstract:
OBJECTIVETo study whether the ethanol extract of Phellinus merrillii (EPM) has chemopreventive potential against liver carcinogenesis.
METHODSThirty male Spraque-Dawley rats were randomly divided into control group, EPM control group, hepatocarcinoma control group, low-dose EPM group and high-dose EPM group, 6 in each group. Using the Solt and Farber protocol in a rat model of hepatocarcinogenesis, the chemopreventive effect of EPM on diethylnitrosamine (DEN)-initiated, 2-acetylaminofluorene (2-AAF) and partial hepatectomy (PH)-promoted liver carcinogenesis in rats was evaluated. Basic pathophysiological and histological examinations, together with the serum levels of glutamic oxaloacetic transaminase (sGOT), glutamic pyruvic transaminase (sGPT) and gamma-glutamyl transpeptidase (γ-GT) were measured.
RESULTSTreatment of EPM at the concentration of 2 g/kg body weight in the diet for 8 weeks clearly prevented the development of carcinogenesis and reduced the levels of sGOT, sGPT, and serum γ-GT of rats as compared with the hepatocarcinoma control group (P<0.05 or P<0.01). These phenotypes were accompanied by a significant increase in natural killer cell activity.
CONCLUSIONEPM showed a strong liver preventive effect against DEN+2-AAF+PH-induced hepatocarcinogenesis in a rat model.