Effects of Shen-Fu Injection () on apoptosis of regulatory T lymphocytes in spleen during post-resuscitation immune dysfunction in a porcine model of cardiac arrest.
- Author:
Wei GU
1
;
Qian ZHANG
2
;
Chun-Sheng LI
3
Author Information
- Publication Type:Journal Article
- Keywords: Chinese medicine; Shen-Fu Injection; cardiopulmonary resuscitation; forkhead/winged helix transcription factor; post-resuscitation immune dysfunction; regulatory T lymphocytes apoptosis
- MeSH: Animals; Apoptosis; drug effects; Cardiopulmonary Resuscitation; Disease Models, Animal; Drugs, Chinese Herbal; administration & dosage; pharmacology; therapeutic use; Forkhead Transcription Factors; genetics; metabolism; Heart Arrest; drug therapy; immunology; pathology; physiopathology; Hemodynamics; drug effects; Injections; Interferon-gamma; metabolism; Interleukin-4; metabolism; Lymphocyte Subsets; drug effects; metabolism; Male; Oxygen; metabolism; RNA, Messenger; genetics; metabolism; Spleen; immunology; Survival Analysis; Swine; Swine, Miniature; T-Lymphocytes, Regulatory; drug effects; immunology
- From: Chinese journal of integrative medicine 2016;22(9):666-673
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate whether Shen-Fu Injection (, SFI) reduces post-resuscitation immune dysfunction in a porcine model of cardiac arrest by modulating apoptosis of regulatory T lymphocytes (Treg) in the spleen.
METHODSAfter 8-min untreated ventricular fibrillation and 2-min basic life support, 24 pigs were divided into 3 groups with a random number table, i.e. SFI group, epinephrine (EP) group, and saline (SA) group (8 in each group), which received central venous injection of SFI (1.0 mL/kg), EP (0.02 mg/kg) and SA, respectively. The same procedure without CA initiation was achieved in the sham-operated (sham) group (n=6). After successful return of spontaneous circulation (ROSC), apoptosis rate of splenic Treg was detected by flow cytometry; and the mRNA expression of forkhead/winged helix transcription factor (Foxp3) of splenic Treg was detected by real time-polymerase chain reaction; and the levels of interleukin-4 (IL-4) and interferon-γ (IFN-γ) in porcine splenic Treg were detected by using enzyme-linked immunosorbent assay (ELISA).
RESULTSCompared with the sham group, the apoptosis rate of Treg was significantly decreased, and the levels of Foxp3 mRNA expression, IFN-γ, IL-4 and IFN-γ/IL-4 were increased in the SA group (P<0.05 or P<0.01). Compared with the EP and SA groups, SFI treatment increased the apoptosis rate of Treg and reduced the levels of Foxp3 mRNA expression, IFN-γ and IFN-γ/IL-4 (P<0.05).
CONCLUSIONSSFI has signifificant effects in attenuating post-resuscitation immune dysfunction by modulating apoptosis of Treg in the spleen.
