Bushen Qiangji Granule () medicated serum inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by inhibiting the BMP/Smads signal pathway in vitro.
- Author:
Hong-Xiao LIU
1
;
Nan JIANG
2
;
Hui-Ying LIANG
2
;
Ying-Yan ZHOU
2
;
Xing-Hua FENG
2
;
Xiao-Yan FENG
3
;
He-Qiu ZHANG
3
;
Zhi-Kui WU
2
;
Quan JIANG
2
;
Jiao FU
2
;
Xiao-Juan MA
2
;
Peng CHEN
2
Author Information
- Publication Type:Journal Article
- Keywords: Bushen Qiangji Granule medicated serum; ankylosing spondylitis; fifibroblasts; ossifification
- MeSH: Adult; Bone Morphogenetic Proteins; metabolism; Cell Differentiation; drug effects; Core Binding Factor Alpha 1 Subunit; genetics; metabolism; Drugs, Chinese Herbal; pharmacology; Fibroblasts; drug effects; metabolism; pathology; Humans; Middle Aged; Osteogenesis; drug effects; genetics; Phosphorylation; drug effects; RNA, Messenger; genetics; metabolism; Serum; metabolism; Signal Transduction; drug effects; Smad Proteins; metabolism; Spondylitis, Ankylosing; genetics; pathology; Young Adult
- From: Chinese journal of integrative medicine 2016;22(11):817-822
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the mechanism of Bushen Qiangji Granule (, BSQJ) in restraining the osteogenic differentiation of ankylosing spondylitis (AS) fifibroblasts.
METHODSHip joint capsules were obtained from AS patients (n=10) receiving total hip replacement and healthy hip joint capsules from patients with hip fracture (n=10) receiving surgery as a control. Finite fifibroblast lines were established from these tissue samples to observe the effect of BSQJ on suppressing osteogenic differentiation of fifibroblasts. The expression of osteogenic marker gene corebinding factor a1 (Cbfa1) and Smad family proteins were examined by Western blot and real-time quantitative polymerase chain reaction (qPCR).
RESULTSThe mRNA expression level of Cbfa1 was significantly higher in AS fibroblasts than that in normal fibroblasts and the expression of pSmad1, pSmad5, Smad4 and Cbfa1 in AS fibroblasts was also higher, demonstrating the activation of the BMP/Smads signal pathway in AS fifibroblasts. BSQJ-medicated serum not only restrained the mRNA and protein expression levels of Cbfa1 and inhibited protein expression level of Smad4 but also decreased the expression quantities of pSmad1 and pSmad5.
CONCLUSIONSBSQJ can inhibit osteogenic differentiation of AS fifibroblasts in vitro by suppressing the activation of the BMP/Smads signal pathway. This may be the important molecular mechanism of BSQJ in regulating AS ossifification.
