Regulatory effect of nerve growth factor on release of substance P in cultured dorsal root ganglion neurons of rat.
- Author:
Xiang-Dong YANG
1
;
Zhen LIU
;
Hua-Xiang LIU
;
Li-Hong WANG
;
Chun-Hong MA
;
Zhen-Zhong LI
Author Information
- Publication Type:Journal Article
- MeSH: Analgesics, Non-Narcotic; pharmacology; Animals; Capsaicin; pharmacology; Cells, Cultured; Dose-Response Relationship, Drug; Embryo, Mammalian; Ganglia, Spinal; cytology; Gene Expression Regulation; drug effects; Nerve Growth Factor; pharmacology; Neurons; drug effects; RNA, Messenger; metabolism; Radioimmunoassay; methods; Rats; Rats, Wistar; Substance P; genetics; metabolism
- From:Neuroscience Bulletin 2007;23(4):215-220
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the regulatory effects of nerve growth factor (NGF) on basal and capsaicin-induced release of neuropeptide substance P (SP) in primary cultured embryonic rat dorsal root ganglion (DRG) neurons.
METHODSDRGs were dissected from 15-day-old embryonic Wistar rats. DRG neurons were dissociated and cultured, and then exposed to different concentrations of NGF (10 ng/mL, 30 ng/mL, or 100 ng/mL) for 72 h. The neurons cultured in media without NGF served as control. RT-PCR were used for detecting the mRNAs of SP and vanilloid receptor 1 (VR1) in the DRG neurons. The SP basal and capsaicin (100 nmol/L)-induced release in the culture were measured by radioimmunoassay (RIA).
RESULTSSP mRNA and VR1 mRNA expression increased in primary cultured DRG neurons in a dose-dependent manner of NGF. Both basal release and capsaicin-evoked release of SP increased in NGF-treated DRG neurons compared with in control group. The capsaicin-evoked release of SP also increased in a dose-dependent manner of NGF.
CONCLUSIONNGF may promote both basal release and capsaicin-evoked release of SP. NGF might increase the sensitivity of nociceptors by increasing the SP mRNA or VR1 mRNA.
