Influences of dibutyryl cyclic adenosine monophosphate and forskolin on human sperm motility in vitro.
- Author:
Ji-Hong LIU
1
;
Yang LI
;
Zheng-Guo CAO
;
Zhang-Qun YE
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Bucladesine; administration & dosage; pharmacology; Colforsin; administration & dosage; pharmacology; Dose-Response Relationship, Drug; Humans; In Vitro Techniques; Male; Osmolar Concentration; Sperm Motility; drug effects
- From: Asian Journal of Andrology 2003;5(2):113-115
- CountryChina
- Language:English
-
Abstract:
AIMTo study the influences of dibutyryl cyclic adenosine monophosphate (dbcAMP) and forskolin on human sperm motility in vitro.
METHODSSemen samples, aseptically obtained by masturbation and prepared by swim-up technique from 20 fertile men, were incubated with different concentrations of dbcAMP and forskolin at 37 deg. Measurements were carried out after 10 min, 20 min, 30 min and 60 min incubation. Motility parameters were estimated by using an automatic analyzing system.
RESULTSTreatment with dbcAMP or forskolin resulted in a significant increase in sperm motility and progressive motility. The larger the concentrations of dbcAMP or forskolin, the greater the effect appeared. The straight linear velocity and curvilinear velocity were not affected by both agents.
CONCLUSIONdbcAMP and forskolin increase the motility and progressive motility of human sperm in vitro.
