Tamoxifen induced multinucleated cells (symplasts) and distortion of seminiferous tubules in rat testis.
- Author:
Urban J A D'SOUZA
1
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents, Hormonal; toxicity; Dose-Response Relationship, Drug; Giant Cells; drug effects; ultrastructure; In Vitro Techniques; Male; Rats; Rats, Wistar; Seminiferous Tubules; cytology; drug effects; Tamoxifen; toxicity; Testis; cytology; drug effects
- From: Asian Journal of Andrology 2003;5(3):217-220
- CountryChina
- Language:English
-
Abstract:
AIMTo evaluate the effect of tamoxifen citrate on male reproductive system of rat.
METHODSGroups of male rats were gavaged with tamoxifen at doses of 200 mg x kg(-1) x d(-1), 400 mg x kg(-1) x d(-1) or 800 mg x kg(-1) x d(-1) in 0.1 mL olive oil for 10 consecutive days. Controls were treated with 0.1 mL olive oil. Rats were anesthetized and killed on d 3, d 15 or d 35 after the last dose. Testes were collected, processed for paraffin embedding, sectioned at 5 microm thickness, stained with HE and analyzed microscopically.
RESULTSThere was a dose-dependent increase in the occurrence of seminiferous tubular distortion with germinal cell sloughing. The highest dose increased the number of multinucleated giant cells on d 3 and d 15.
CONCLUSIONTamoxifen citrate induces multinucleated giant cells and germinal epithelial sloughing in a dose-dependent manner and these changes are detrimental to male fertility.
