Genic and non-genic regulation of low frequence pulsed electromagnetic fields on osteoblasts differentiation.
- Author:
Qingqing FANG
1
;
Zhizhong LI
2
;
Jian ZHOU
1
;
Wengui SHI
1
;
Juanli YAN
1
;
Keming CHEN
3
Author Information
1. Institute of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
2. College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China.
3. Institute of Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China. chenkm@lut.cn.
- Publication Type:Journal Article
- MeSH:
Alkaline Phosphatase;
metabolism;
radiation effects;
Animals;
Cell Differentiation;
genetics;
radiation effects;
Cells, Cultured;
Core Binding Factor Alpha 1 Subunit;
metabolism;
radiation effects;
Electromagnetic Fields;
Osteoblasts;
chemistry;
radiation effects;
Osteogenesis;
genetics;
radiation effects;
Rats;
Transcription Factors;
metabolism;
radiation effects
- From:
Journal of Zhejiang University. Medical sciences
2016;45(6):568-574
- CountryChina
- Language:Chinese
-
Abstract:
To study the genic and non-genic regulation of 50 Hz 0.6 mT pulsed electromagnenic fields (PEMF) on rat calvarial osteoblasts (ROB) differentiation.ROBs were achieved by enzyme digestion, and treated with 50 Hz 0.6 mT PEMFs for 1.5 hours after subculture. The alkaline phosphatase (ALP) activity, mRNA transcription of ALP, Runx2 and OSX and protein expression of Runx2 and OSX were detected at 0, 3, 6, 9 and 12 hours after PEMF treatment.The ALP activity at 3 hours after treatment was significantly higher than that in the control(<0.01), while the mRNA transcription of ALP began to increase at 6 hours after treatment. The mRNA transcription of Runx2 increased immediately after treatment and regressed at 6 hours, then increased again. The protein expression of it corresponded but with a little lag. The mRNA transcription of OSX also raised instantly after treatment, then returned to the level of control at 6 hours, and lower than control at 12 hours significantly. The protein expression of it also corresponded but with a bit delay.There are genic regulation for the protein expression of Runx2 and OSX, and non-genic regulation for the ALP activity on the process of 50 Hz 0.6 mT PEMFs prompts ROBs differentiation.
